fibroblast growth factor 2  (R&D Systems)

Journal: Journal of Applied Oral Science

Article Title: Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis

doi: 10.1590/1678-775720150606

Figure Lengend Snippet: Proliferation assay of MC3T3-E1 cells at different concentrations of FGF-2. Cell growth of MC3T3-E1 cells was examined on monolayer culture using MTT assay at different concentrations of FGF-2 (0, 2, 20,100 µg/ml). The high proliferative potential of MC3T3-E1 cells was observed in the presence of 20 µg/ml of FGF-2 at day 5. Statistical significance of p<0.01 is indicated by ***

Article Snippet: Mouse recombinant FGF-2 (Sigma-Aldrich) was used to evaluate the optimum concentration of FGF-2 needed for cell proliferation, therefore the samples were subjected to different concentrations of FGF-2 (2, 20, 100 µg/ml) .

Techniques: Proliferation Assay, MTT Assay

Journal: Journal of Applied Oral Science

Article Title: Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis

doi: 10.1590/1678-775720150606

Figure Lengend Snippet: Proliferation assay of IP-CHA/MC3T3-E1 cells under the influence of FGF-2 and/or melatonin (MEL). Cell growth was significantly induced by FGF-2 treatment compared to the control and to the treatment with MEL. The combination of MEL and FGF-2 more markedly promoted the proliferation of IP-CHA/MC3T3-E1 cells. Statistical significances shown by p<0.05 and p<0.01 are indicated by * and **, respectively

Article Snippet: Mouse recombinant FGF-2 (Sigma-Aldrich) was used to evaluate the optimum concentration of FGF-2 needed for cell proliferation, therefore the samples were subjected to different concentrations of FGF-2 (2, 20, 100 µg/ml) .

Techniques: Proliferation Assay

Journal: Journal of Applied Oral Science

Article Title: Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis

doi: 10.1590/1678-775720150606

Figure Lengend Snippet: mRNA expressions of osteopontin (OPN) and osteocalcin (OCN) of IP-CHA/MC3T3-E1 cells under the influence of FGF-2 and/or melatonin (MEL). mRNA expressions of OPN and OCN were significantly enhanced by treatment with MEL from day 3 compared to the control and to the treatment with FGF-2. The effect of combined MEL and FGF-2 was significantly greater. Statistical significances shown by p<0.05 and p<0.01 are indicated by * and **, respectively

Article Snippet: Mouse recombinant FGF-2 (Sigma-Aldrich) was used to evaluate the optimum concentration of FGF-2 needed for cell proliferation, therefore the samples were subjected to different concentrations of FGF-2 (2, 20, 100 µg/ml) .

Techniques:

Journal: Journal of Applied Oral Science

Article Title: Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis

doi: 10.1590/1678-775720150606

Figure Lengend Snippet: ALP enzyme activity of IP-CHA/MC3T3-E1 cells under the influence of FGF-2 and/or melatonin (MEL). ALP activity from day 3 was significantly higher in cells treated with MEL compared to the control and to those treated by FGF-2. The combination of MEL and FGF-2 markedly increased that activity. The ratio of absorbance of each sample in relation to the control sample at day 3 was observed. Statistical significances shown by p<0.05 and p<0.01 are indicated by * and **, respectively

Article Snippet: Mouse recombinant FGF-2 (Sigma-Aldrich) was used to evaluate the optimum concentration of FGF-2 needed for cell proliferation, therefore the samples were subjected to different concentrations of FGF-2 (2, 20, 100 µg/ml) .

Techniques: Activity Assay

Journal: Journal of Applied Oral Science

Article Title: Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis

doi: 10.1590/1678-775720150606

Figure Lengend Snippet: Alizarin Red Staining of MC3T3-E1 cells under the influence of FGF-2 and/or melatonin (MEL). a- Alizarin Red staining of monolayer cultures showed increased mineralization after 2 weeks of treatment with MEL or FGF-2 in osteogenic induction medium compared to the control. The combination of MEL and FGF-2 resulted in more intense staining; b- Quantified values obtained from cells cultured within IP-CHA constructs suggested a role for MEL in mineralization, while combined FGF-2 and MEL induced increased mineralization. Statistical significance shown by p<0.05 is indicated by *

Article Snippet: Mouse recombinant FGF-2 (Sigma-Aldrich) was used to evaluate the optimum concentration of FGF-2 needed for cell proliferation, therefore the samples were subjected to different concentrations of FGF-2 (2, 20, 100 µg/ml) .

Techniques: Staining, Cell Culture, Construct